Maybe, and yes.
The thing is different pheromones have different rates at which they dissipate from the skin. There are some estimations of pheromone-in-alcohol-spray half life by MarkL:
sorry, it is mainly focused on the ones men wear, but you get the idea. Ishine doesn’t last that long and may require periodical top-ups if the situation needs it. -none, which you probably won’t be wearing, lasts for ages; I don’t know about copulins and their half-life. You may develop a feeling for when hits are wearing off and self effects and all that, and might reapply. Or, you might have made such a good impression initially with the pheros that you can get on without reapplying; just let the momentum of the day’s events carry you along…