Hi geo_c, welcome to the this active forum.
First of all, could you tell me what pheromone did you use before?
Actually A314 is an oil based pheromone mix, and it usually last for about 12 hours.
Yes, it you completely wash it away, then there will be no more effect. And I would recommend trying each product by itself to find your sweet spot first before moving to mixing it other pheoros. You can read How to find your sweet spot… to find out more.
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Waiting for your reply!